abstract：:Age-associated thymic involution manifests its effects in a variety of ways that are related to a loss of T cell function. These include the appearance of a non-functional subset of T cells that increase in representation with age. Moreover there is a loss of T cell proliferative ability, a decline in the synthesis an...

journal_title：Experimental gerontology

authors： Song L,Kim YH,Chopra RK,Proust JJ,Nagel JE,Nordin AA,Adler WH

更新日期：1993-07-01 00:00:00

abstract：:Androgen levels decrease with aging in men. Androgen deficiency in elderly men may lead to asthenia, decrease in muscle mass, osteoporosis, decrease in sexual activity, and, in some cases, changes in mood and cognitive function. Combination of these factors may result in impaired quality of life in the elderly male. A...

journal_title：Experimental gerontology

authors： Swerdloff RS,Wang C

更新日期：1993-07-01 00:00:00

abstract：:In the present study, using RNA gel-blot analysis, we characterized the developmental changes in the prevalence of mRNA coding for fibronectin (FN), glial fibrillary acidic protein (GFAP), neurotrophic protein S100 beta, and beta-actin mRNA in rat hippocampus and forebrain from 1 to 720 days of age. We found that the ...

journal_title：Experimental gerontology

authors： Wagner AP,Reck G,Platt D

更新日期：1993-03-01 00:00:00

abstract：:We have developed a simian virus 40 (SV40) T-antigen immortalized human cell line, 1MR90-D305.2H4 (IDH4), in which the expression of T-antigen is controlled by the mouse mammary tumor virus (MMTV) promoter and thus regulated by steroids such as dexamethasone. Studies on the regulation of proliferation by T-antigen led...

journal_title：Experimental gerontology

authors： Shay JW,West MD,Wright WE

更新日期：1992-09-01 00:00:00

abstract：:Caloric restriction (CR) without malnutrition in mice and rats reduces the incidence of spontaneous tumors and delays their appearance while increasing maximum life span. These results depend largely on CR per se, and not on low intakes of fat or other nutrients. Although most studies have tested CR imposed early in l...

journal_title：Experimental gerontology

authors： Weindruch R

更新日期：1992-09-01 00:00:00

abstract：:Human diploid lung fibroblasts (IMR-90) were used to investigate the reported increase in beta-adrenergic-stimulated cyclic adenosine 3',5'-monophosphate (cAMP) levels in fibroblasts aged in culture. Under basal conditions cellular cAMP was 34.2 +/- 5.6 and 38.4 +/- 9.1 pmol/mg protein in early (PDL 22-24) and late (P...

journal_title：Experimental gerontology

authors： Ethier MF,Medeiros M,Romano FD,Dobson JG Jr

更新日期：1992-01-01 00:00:00

abstract：:Several papers reported in recent years on a change in the age population distribution of the circulating erythrocytes in old mice, rats, rabbits, and humans. The results indicate the presence of a chronologically younger cell population in old animals and humans. The cells are typically lower in density and larger. I...

journal_title：Experimental gerontology

authors： Danon D,Bologna NB,Gavendo S

更新日期：1992-01-01 00:00:00

abstract：:It has been often demonstrated that during senescence some neurons undergo atrophic changes while others add new processes and terminals. Because microtubules form a substantial component of the dendritic and axonal cytoskeleton, we have studied the amount of tubulin and acetylated alpha-tubulin in three young (6 mont...

journal_title：Experimental gerontology

authors： Fifkova E,Morales M

更新日期：1992-01-01 00:00:00

abstract：:The ascorbic acid content of Drosophila melanogaster was found to be high in the absence of a dietary source. The amount of ascorbic acid per fly declined with aging in both the Oregon R and Swedish C strains. The median life span at 25 degrees C was 45 days for Swedish C and 59 days for Oregon R. The amount of ascorb...

journal_title：Experimental gerontology

authors： Massie HR,Shumway ME,Whitney SJ,Sternick SM,Aiello VR

更新日期：1991-01-01 00:00:00

abstract：:A longitudinal study was performed to confirm the inverse relationship between in vivo age and in vitro proliferative capacity previously observed in a cross-sectional study, and to investigate the relationship between the growth of dermal fibroblasts in vitro and a physiological function (i.e., wound repair) that is ...

journal_title：Experimental gerontology

authors： Bruce SA,Deamond SF

更新日期：1991-01-01 00:00:00

abstract：:A mathematical model of cellular metabolism is used to relate the rates of cell division and waste production to the concentrations of oxygen and glucose in the medium in which a normal diploid cell culture is grown. The metabolic model in tandem with an earlier waste-content model based on the waste-product theory of...

journal_title：Experimental gerontology

authors： Hirsch HR,Witten M

更新日期：1991-01-01 00:00:00

abstract：:The loss of bone mineral with aging and subsequent development of osteoporosis is a common problem in elderly women, and as life expectancy increases, in elderly men as well. Space flight also causes bone loss and could be a limiting factor for long duration missions, such as, a Mars expedition or extended occupation ...

journal_title：Experimental gerontology

authors： LeBlanc A,Schneider V

更新日期：1991-01-01 00:00:00

abstract：:The effect of aging was tested on experimental ventricular arrhythmias in isolated heart preparations from normal Wistar rats (NWR), Wistar Kyoto rats (WKY), and spontaneously hypertensive rats (SHR). Delayed afterdepolarizations and triggered activity induced by high-calcium perfusion (16 mM) in isolated papillary mu...

journal_title：Experimental gerontology

authors： Carbonin PU,Di Gennaro M,Pahor M,Bernabei R,Sgadari A,Gambassi G Jr

更新日期：1990-01-01 00:00:00

abstract：:In order to establish the role of calcitonin (CT) in postmenopausal bone loss, we studied CT metabolism in 25 pre- and postmenopausal women. Postmenopausal women presented a highly significant reduction of CT basal levels compared to premenopausal females (p less than 0.01). Also, production rates of CT in osteoporoti...

journal_title：Experimental gerontology

authors： Reginster JY,Deroisy R,Lecart MP,Sarlet N,Fontaine MA,Albert A,Franchimont P

更新日期：1990-01-01 00:00:00

abstract：:It is well established that during early life the thymus gland and the neuroendocrine system influence each other's maturation. Furthermore, there is a growing body of evidence indicating that the immune and neuroendocrine systems also function as a bidirectional network during adult life. In order to assess possible ...

journal_title：Experimental gerontology

authors： Goya RG,Naylor PH,Goldstein AL,Meites J

更新日期：1990-01-01 00:00:00

abstract：:To determine whether age-related differences in body weight loss in hyperthyroidism could be related to caloric intake, the body weight and food consumption of Fischer 344 male rats were monitored every other day for four weeks. Six-month-old (young) rats were compared to 16-month-old rats (intermediate age) and 25-mo...

journal_title：Experimental gerontology

authors： Mooradian AD

更新日期：1990-01-01 00:00:00

abstract：:The content and synthesis of GABA were measured in the cortex and striatum of young adult (4 months), mature (14 months), and old (24 months) male Wistar rats. GABA synthesis was determined from the GABA accumulation induced by aminooxyacetic acid (AOAA). Aging did not affect GABA content in the cortex (1.03 +/- 0.04 ...

journal_title：Experimental gerontology

authors： Carfagna N,Moretti A

更新日期：1990-01-01 00:00:00

abstract：:A colony of male Lakeview Golden (LVG) Syrian hamsters has been maintained for the last nine years as a source of various tissues for cellular aging studies. Observations on this colony also yielded data on survival time and physical and pathological manifestations of aging in this strain. Based on 150 spontaneous dea...

journal_title：Experimental gerontology

authors： Deamond SF,Portnoy LG,Strandberg JD,Bruce SA

更新日期：1990-01-01 00:00:00

abstract：:Neuroblastoma cells (N1E-115) were used as models of transient (T) and long-lasting (L) Ca++ channels. The whole cell version of the patch clamp technique was used to measure inward Ca++ currents, and the fluorescent indicator, Fura-2, was used to measure changes in intracellular Ca++. Cells were cultured and selected...

journal_title：Experimental gerontology

authors： Pang PK,Wang R,Wu LY,Karpinski E,Shan J,Benishin CG

更新日期：1990-01-01 00:00:00

abstract：:We have isolated a senescence-specific clone (pSEN) from a cDNA library constructed from late passage WI-38 human diploid fibroblast that accounts for approximately 1% of the recombinants. Nucleotide sequence analysis of the partial cDNA clone has led to the identification of pSEN as elongation factor I alpha. Norther...

journal_title：Experimental gerontology

authors： Giordano T,Kleinsek D,Foster DN

更新日期：1989-01-01 00:00:00

abstract：:The accuracy of protein synthesis has been measured in extracts from human diploid fibroblasts of different ages. Extracts were supplied with purified mRNA for the coat protein of the cowpea variant of tobacco mosaic virus (CcTMV), which lacks codons for cysteine and methionine. The presence of 35S-cysteine in CcTMV c...

journal_title：Experimental gerontology

authors： Luce MC,Bunn CL

更新日期：1989-01-01 00:00:00

abstract：:Based on evidence that human diploid fibroblasts (HDF) from the Werner syndrome (WS) of premature aging might overexpress an inhibitor of DNA synthesis (IDS), we prepared a eukaryotic cDNA expression library from WS mRNA and tested it for IDS activity in a transient assay. Two of six WS cDNA pools tested gave IDS acti...

journal_title：Experimental gerontology

authors： Goldstein S,Murano S,Benes H,Moerman EJ,Jones RA,Thweatt R,Shmookler Reis RJ,Howard BH

更新日期：1989-01-01 00:00:00

abstract：:The age-dependent modifications of both basal and lymphokine-induced natural killer (NK) activity were analyzed in Balb/c inbred mice by measuring either the percentage of specific lytic activity in a 51 Cr release assay or the percentage of cells capable of binding the YAC-1 target cells. The basal lytic activity of ...

journal_title：Experimental gerontology

authors： Provinciali M,Muzzioli M,Fabris N

更新日期：1989-01-01 00:00:00

abstract：:The gangliosides in human diploid fibroblasts--TIG-1, TIG-7, and IMR-90--were analysed at different cell densities at early and late passages to clarify the relationship between age and cell density dependent changes of the gangliosides. In early passages, the ganglioside concentrations increased with increase in cell...

journal_title：Experimental gerontology

authors： Ohsawa T

更新日期：1989-01-01 00:00:00

abstract：:A micromethod was used to study myelin from individual rat optic nerves as a function of age. Protein and galactolipid content as well as 2',3'-cyclic nucleotide phosphohydrolase (CNPase) activity at ages 2, 4, 22, and 28 months were determined. Protein content increased significantly at 22 months and decreased at adv...

journal_title：Experimental gerontology

authors： Jacks AS,Richardson AR,Jones MA

更新日期：1989-01-01 00:00:00

abstract：:The goal of these experiments was to determine if the increased longevity characteristics of our genetically selected long lived line of Drosophila could be attributed to metabolic differences. The data shows an inverse relationship between life span and temperature for both the long lived (L) and normal (R) strains; ...

journal_title：Experimental gerontology

authors： Arking R,Buck S,Wells RA,Pretzlaff R

更新日期：1988-01-01 00:00:00

abstract：:In this study, spontaneous multiple unit activity (MUA, action potentials derived simultaneously from a number of neurons in a given brain region) was recorded through electrodes chronically implanted in the parietal cerebral cortex of the rats of 1, 3, 6, 9, 12, and 26 months of age (cross-sectional study). Electroph...

journal_title：Experimental gerontology

authors： Roy D,Singh R

更新日期：1988-01-01 00:00:00

abstract：:While it may be possible to employ panels of molecular parameters which correlate with senescence in vivo or in vitro, in a manner analogous to the use of mutagenesis assays for economic carcinogen screening, such an endeavor would at present be impeded by the absence of a clear mechanistic rationale for focusing on p...

journal_title：Experimental gerontology

authors： Shmookler Reis RJ

更新日期：1988-01-01 00:00:00

abstract：:Long-lived recombinant inbred lines, some of which have mean and maximum life spans up to 70% longer than wild type, were used in these analyses. Longer life results from a slower exponential rate of increase in mortality. General motor activity decreases with chronological age in all genotypes; this decay in general ...

journal_title：Experimental gerontology

authors： Johnson TE,Conley WL,Keller ML

更新日期：1988-01-01 00:00:00

abstract：:Light and electron microscope comparisons were made of parotid and submandibular glands from male Swiss-Webster white mice 3, 13, and 18 months old. The glands from the 13- and 18-month-old mice were less organized and the parenchyma was not as dense. Fibrous connective tissue, intracellular lipofuscin granules, and r...

journal_title：Experimental gerontology

authors： Meisel DL,Skobe Z,Prostak KS,Shklar G

更新日期：1988-01-01 00:00:00

abstract：:Serum lipid peroxide (LPO) in 72 healthy male subjects was studied using a newly developed assay method based on the reaction of LPO with methylene blue derivative (MCDP; 10-N-Methylcarbamoyl-3,7-dimethylamino-10 H-phenothiazine). This method is specific for lipid hydroperoxide and the relation is equimolar. The value...

journal_title：Experimental gerontology

authors： Tateishi T,Yoshimine N,Kuzuya F

更新日期：1987-01-01 00:00:00

abstract：:At 11 and 30 degrees C there was no change in the amount or in the buoyant density of nuclear DNA of male Oregon R Drosophila melanogaster with aging. When normal flies were maintained at 11 degrees C (at which temperature they do not fly), mitochondrial DNA content declined gradually with aging and 39.4% of the origi...

journal_title：Experimental gerontology

authors： Massie HR,Williams TR

更新日期：1987-01-01 00:00:00

abstract：:Fetal and placental weights and peripheral blood progestagen concentrations were examined on days 13, 16 and 22 of gestation in young (3-5 month) nulliparous and old (9-12 month) multiparous, albino Wistar rats. There was no difference in fetal weights at any stage examined whereas placental weights were significantly...

journal_title：Experimental gerontology

authors： Rahima A,Bruce NW

更新日期：1987-01-01 00:00:00

abstract：:Cardiovascular function during sleep was studied in 14 (6 males, 8 females) healthy, active, older adults (57-70) years). Noninvasive measures of stroke volume (SV), heart rate (HR), cardiac output (CO), pre-ejection period (PEP), and left ventricular ejection time (LVET) were taken using impedance cardiography and co...

journal_title：Experimental gerontology

authors： Bevier WC,Bunnell DE,Horvath SM

更新日期：1987-01-01 00:00:00

abstract：:The adult blowfly, Phormia regina, reveals a substantial homeostatic capability when examined for its ability to regulate externally administered additions to its blood sugar. As well, results of experiments done at intervals throughout the entire adult life of the fly show that there is no decrease over time in the e...

journal_title：Experimental gerontology

authors： Anelli CM,Friedman S

更新日期：1986-01-01 00:00:00

abstract：:The immune response declines with age. This decline correlates with thymic involution and involves primarily a loss in T-cell function, whereas humoral immunity is more variably affected. In the current experiments we have measured immunoglobulin synthesis in vitro after mitogen stimulation, and specific antibody resp...

journal_title：Experimental gerontology

authors： Ershler WB,Robbins DL,Moore AL,Hebert JC

更新日期：1986-01-01 00:00:00

abstract：:Like the weather, many people talk about aging, but no one seems able to do anything about it. This implacability derives, at least in part, from our inability to provide a conceptual base from which analytic insight might derive. The emerging field of nonlinear thermodynamics presents a new view of the universe. It r...

journal_title：Experimental gerontology

authors： Bortz WM 2nd

更新日期：1986-01-01 00:00:00

abstract：:The alterations of the lipid composition and fluidity of liver plasma membranes as well as the activity of membrane-bound phospholipase A2 (EC 3.1.1.4) in male and female rats of different ages are investigated. An age-induced increase of the protein/phospholipid and cholesterol/phospholipid ratios is observed, as wel...

journal_title：Experimental gerontology

authors： Petkova DH,Momchilova AB,Koumanov KS

更新日期：1986-01-01 00:00:00

abstract：:Male F344 rats, 3, 12, and 24 months of age, were acclimated to either 5 degrees C (CA) or 23 degrees C (non-CA). At the end of acclimation, all CA rats exhibited higher basal oxygen uptake than the non-CA when measured at 28 degrees C. When challenged by a 3-hour exposure to -10 degrees C, significant difference in t...

journal_title：Experimental gerontology

authors： Kiang-Ulrich M,Horvath SM

更新日期：1985-01-01 00:00:00

abstract：:Effects of glucagon and epinephrine stimulation on urea synthesis from lactate, pyruvate, NH4Cl, and ornithine in isolated rat hepatocytes from young and old rats were compared. It was found that the production of urea from those substrates was almost the same in both age groups. The concentrations of hormones that ca...

journal_title：Experimental gerontology

authors： Kmieć Z,Myśliwski A

更新日期：1985-01-01 00:00:00